Our key scientific goal at the Esposito lab is the pursuit of an integrated understanding of the molecular, cellular and physiological mechanisms by which select plant-derived proteins and phytoactives may modulate tissue regeneration and repair. Our research has encompassed the disciplines of plant chemistry, inflammation, skin biology, muscle physiology, basic and applied molecular and cellular biology and nutrition. The research has spanned both the basic and clinical sciences.
Thus, our interdisciplinary approach allows for a comprehensive evaluation of plant-derived molecules by looking at multiple cellular mechanisms of action to understand the systemic architecture that controls muscle growth, muscle stem (satellite) cells differentiation, inflammation, skin cell migration and wound closure. The lab thrives on our enthusiasm of interdisciplinary work, devotion to research, and leadership at the NC State University Plants for Human Health Institute. We are constantly attracting new students and research scientists to our group, resulting in steady increase of output and visibility of complex biological systems research at NC State University.
Our current goal employs these skills and expertise to develop an interdisciplinary, broad-based tissue regeneration and repair research program that will apply basic science discoveries in the areas of phytochemistry, regenerative medicine, and skin care to improve human health and wellbeing. We are heavily focus on the application of plant-based bioactives to three interconnected research areas:
- Inflammation and tissue regeneration as it applies to wound healing and skin care;
- Cell-based biological screening in conventional, 3D matrices, and stem culture technologies; and
- Muscle aging and repair.
Development of phytochemical-rich watermelon particles
Investigator: Roberta Correia, Visiting Scholar
The primary objective of our project is to produce lycopene/citrulline-rich particles using watermelon as our primary source of phytochemicals. An efficient spray drying process will be developed and the particles will function as botanical additives to multiple food and cosmetic applications. In preliminary experiments, semi purified watermelon extracts with a high concentration of lycopene/citrulline extracts have been obtained. In this next phase, our goal is to obtain spray dried lycopene/citrulline-rich particles by complexing different drying carriers (protein and complex carbohydrates: gelatin, whey protein isolate and soy protein isolate; maltodextrin) with watermelon extracts. In fact, proteins have been extensively used for industry applications, and they act as efficient emulsifiers that enhance the bioavailability of phytochemicals, and stabilize the interface in oil-in-water (O/W) emulsions, besides increasing the drying efficiency. On the other hand, maltodextrin have been widely used in the industry for several purposes. This strategy aims to obtain watermelon/protein particles with preserved and concentrated lycopene and citrulline contents. In order to achieve our goal, the drying process will be evaluated and the best drying parameters will be determined. Besides this, the physicochemical attributes, phytochemical content and in vitro biological activities (inflammatory markers and wound healing activity) of watermelon/protein particles will be determined and compared.
Dietary Polyunsaturated Fatty Acids and Immune Response to Respiratory Pathogens
Investigator: Kathleen Walter
This project uses neonatal swine as our model, which has translational applications to both the swine industry and human health. The main goals of this project are: (i) to evaluate immune cell fatty acid enrichment from dietary supplementation with different long-chain polyunsaturated fatty acids (PUFA); and (ii) to study the effects of PUFA enrichment in innate immune cells to respiratory pathogens ex vivo.
The Effect of Brassicaceae Bioactive Compounds on Biomarkers of Inflammatory Processes Associated with Aging
Investigator: Sierra Bonney
The focus of this project is to further study bioactive compounds found within Brassicaceae plants in order to identify health benefits both in cell models and an accelerated aging mouse model. Brassinosteroids found within these plants have been found to have a number of health promoting properties including increased lean muscle mass, physical fitness, anti-inflammatory properties, and acceleration of cutaneous wound healing. By using an accelerated aging model, applications of these phytochemicals on inflammatory diseases and age-related chronic inflammation can be evaluated.
Stimulation of wound healing and muscle growth function by Brassinosteroids
This project utilizes a systemic architecture that controls inflammation and tissue regeneration processes and investigate brassinosteroids with novel health benefits, especially in regards to fundamental and applied relevance to skin care and muscle health.